Bin2 phosphorylation mutant

Author: dbyq

August undefined, 2024

WebApr 11, 2024 · Highlights. As a major environmental factor impacting the seasonal growth and geographical distribution of plants, temperature change significantly affects crop quality and productivity. Ethylene is a gaseous hormone with an important role in plant growth, development, and multiple stress responses, including heat and cold. Temperature stress ... WebBIN2-mediated phosphorylation of ARF7 and ARF19 suppresses their interaction with AUX/IAAs, and subsequently enhances the transcriptional activity to their target genes …

Histone deacetylase HDA6 enhances brassinosteroid signaling by ... - PNAS

WebJul 11, 2002 · BIN2 encodes a member of the glycogen synthase kinase-3 (GSK3)-like kinases ( 14, 15 ). The increased kinase activity by the semidominant bin2–1 mutation and the phenotypes of transgenic plants … WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced … reach rose 違い

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To further identify the mechanism by which HY5 regulated BIN2 in the light to destabilize BZR1, we first checked whether HY5 regulated the mRNA and/or protein levels of BIN2. As shown in Supplementary Fig. 5, both the mRNA and protein levels of BIN2 were similar in hy5 and WT, suggesting that HY5 does not … See more To investigate the cross-talk between light and BR signaling, we performed yeast two-hybrid analyses and found that BIN2, the key negative … See more Next, we investigated whether HY5 could genetically interact with BIN2 by crossing hy5 into bin2-1, a gain-of-function mutant of BIN228. We found that the hypocotyls of double mutant … See more To understand which amino acid residues of HY5 were important for its interaction with BIN2, we screened BIN2-interacting sites in the HY5 LZ region. As shown in Fig. 5a, changing HY5 Leu137 (L137) to Asp (named HY5-M) … See more Previous reports have suggested that HY5 and BIN2 have similar effects on BZR1 protein accumulation20,27. To gain further insight into their … See more WebIt was shown that the BIN2-catalyzed phosphorylation of BSK3 promotes the interactions of BSK3 with the BR receptor BRI1 (Brassinosteroid-Insensitive1) ... elimination of GSK3-like kinases resulted in an increased ratio of SE/CC whereas overexpression of a gain-of-function mutant variant bin2-1 slightly decreased the SE/CC ratio, ... WebBIN2 is a member of the GSK3-like kinase family, whose members can recognize substrates by either forming a stable protein complex with them or recognizing substrate epitopes that have been previously phosphorylated by an alternative protein kinase in a process known as priming phosphorylation. reach rotherham

The GSK3-like Kinase BIN2 Is a Molecular Switch between

Interplay between phosphorylation and SUMOylation …

WebJan 30, 2024 · Importantly, the BIN2 phosphorylation of Thr280 residue restricts the function of CO in promoting flowering through affecting its DNA-binding activity. Moreover, we … WebThus, BIN2 is a good candidate to regulate PIF4 levels, as inactivation of this kinase by BL may result in the fastest-migrating form of this factor seen in BL-treated seedlings ( Fig. 1C ). A direct interaction of the full-length BIN2 protein and PIF4 was actually observed in yeast two-hybrid assays ( Fig. 2A ). how to start a clean bootWebBIN2 integrates multiple signaling pathways by phosphorylating a range of proteins including the components in light signaling. For example, PIF3, PIF4, and PIF5 can be phosphorylated by BIN2 and then degraded via the 26S proteasome pathway to control transcription and hypocotyl growth ( Bernardo-García et al., 2014; Ling et al., 2024 ). how to start a cleaning business in arizona

"WebNov 1, 2002 · It was hypothesized that, in the absence of BR signals, BIN2 is a constitutively active kinase that would phosphorylate several positive BR-signaling proteins, rendering … " - Bin2 phosphorylation mutant

Bin2 phosphorylation mutant

The GSK3-like kinase BIN2 phosphorylates and destabilizes BZR1, …

WebMar 30, 2024 · The BIN2 gain-of-function mutant bin2-1 displays an enhanced photomorphogenic phenotype in both far-red and red lights. phyA-enhanced accumulation of BIN2 promotes the phosphorylation of BES1 in far-red light. BIN2 acts genetically downstream from PHYA to regulate photomorphogenesis under far-red light. WebBIN2 phosphorylation of RD26 is required for RD26 transcriptional activation on drought-responsive genes. RD26 overexpression suppressed the brassinazole (BRZ) insensitivity of BIN2 triple mutant bin2 bil1 bil2, …

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WebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated … WebOur genetic, biochemical, and physiological assays revealed that despite functional redundancy, BIN2 plays a dominant role among the three group II members in regulating …

WebJul 1, 2024 · BIN2, a loss-of-function mutant of BIN2 and its two closest homologs, was hyposensitive to ABA in primary root inhibition, ABA-responsive gene expression, and phosphorylating ABA Response Element Binding Factor (ABF) 2 fragment by in-gel kinase assays, suggesting that these GSK3-like kinases function as positive regulators in ABA … http://genesdev.cshlp.org/content/28/15/1681.long

WebDec 29, 2024 · BIN2 mutant expression assay The native promoter-driven genomic BIN2 gene (4058 kb) and the corresponding mutated genes were fused with the YFP tag and transferred into a Col-0 background. The … WebBIN2 (brassinosteroid-insensitive 2) kinase, a GSK3-like kinase functioning as a negative regulator in BR signaling, directly phosphorylated RAPTOR1B (regulatory-associated protein of TOR 1B), a substrate-recruiting subunit in the TOR complex, at a conserved serine residue within a typical BIN2 phosphorylation motif.

WebSep 6, 2009 · GST–BIN2 M115A is a BIN2 kinase-inactive mutant. (e) GST–BIN2 or GST–bin2-1 was first treated with BSU1–YFP immunoprecipitated from BR-treated (+ BL; 0.25 μM for 30 min) or untreated 35S ...

WebApr 19, 2002 · To test if BIN2 phosphorylates BES1 or mutant bes1 in vitro, the proteins were expressed in E. coli, purified with GST or MBP tags, respectively, and used in an in vitro phosphorylation assay. BIN2 efficiently phosphorylated BES1 or mutant bes1 to a greater extent than BIN2 autophosphorylation, consistent with 22 putative BIN2 sites in … how to start a church with no moneyWebNov 14, 2024 · The BIN2–POLAR–BASL complex migrates to the cell cortex and polarizes before the ACD, thereby relieving the BIN2 inhibition on SPCH in the nucleus and attenuating the MAPK signalling in the... how to start a cleaning business ebookWebBIN2 is involved in a wide range of developmental processes through phosphorylating a multitude of substrates, including many TFs (Youn and Kim, 2015 ). To test this hypothesis that RD26 is phosphorylated by … how to start a classWebtion, BIN2 phosphorylation stabilizes MYBL2. Such dual role of ... in the mutant (Fig. S1A), as reported previously (45). We created double mutants of mybl2 with bri1-5, a weak loss-of-function allele of BR receptor BRI1 (47), and bes1-D, a gain-of-function mutant in the BR pathway (4). Although the single mutant mybl2 how to start a cleaning business in coloradoWebDa Som Lee1,2 iD Tae Hyeon An2,3 iD Hyunmi Kim2,3 iD Eunsun Jung4 iD Gyeonghun Kim5 iD Seung Yeon Oh6 iD Jun Seok Kim7 iD Hye Jin Chun8 iD Jaeeun Jung9 iD Eun-Woo Lee2,3 iD Baek-Soo Han3,10 iD Dai Hoon Han11 iD Yong-Ho Lee8,12 iD Tae-Su Han3,4 iD Keun Hur13 iD Chul-Ho Lee3,14 iD Dae-Soo Kim9 iD Won Kon Kim2,3 iD Jun … reach router linkWebNov 11, 2024 · e, Western blot with the custom-made p-BIN2 antibody on BIN2-WT or BIN2-AA (T423A and S424A non-phosphorylable mutant)-expressing A2780 cells showed that the p-BIN2 antibody detected an expected ... reach router navigateWebJan 10, 2024 · Bottom schematic shows BASL polarization requires MAPK-mediate protein phosphorylation and the plasma membrane-associated BRX proteins. Unknown regulators (orange) are anticipated to mediate BASL polarization. ... Genotyping results showed that these mutant plants are ... GSK3-like BIN2 kinases 12 and the BSL phosphatases 13, … reach router npm