Ef-1α intron a的功能

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August undefined, 2024

WebEF1 promoter (Ubiquitous) in pDRIVE expression plasmid. The EF-1 alpha gene encoding elongation factor-1 alpha is an enzyme which catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes. EF-1α is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells. WebThe β-tubulin gene intron 3 region and EF-1α intron 4 should prove useful in phylogenetic and epidemiological studies of additional Claviceps species. AB - The intron 3 region of the β-tubulin gene, and intron 4 of the translation elongation factor gene were PCR-amplified, cloned, and sequenced to determine relationships among Claviceps ...

The human elongation factor 1 alpha (EF-1α) first intron highly ...

http://www.inovogen.com/lentivirus/lentivirusvector/pLV-EF1a-EGFP2APuro/ WebAug 1, 2001 · In inspecting the EF-1α intron matrices (see figs. 2 and 3), it is clear that some indel events are phylogenetically informative (e.g., consistent with unambiguous exon signal). If we were to either exclude indel sites or treat gaps as missing, we would be excluding available phylogenetic information. Alternatively, some strings of adjacent ... dating old pepsi bottles

Ef1a是什么? - 百度知道

WebpLV-EF1a-EGFP(2A)Puro载体是基于HIV1的慢病毒基因过表达载体，采用EF1a启动子启动目的基因的表达。EF1a启动子来源于EF1a启动子来自人类靶向延长因子 1α（EF1A）基因，可在多数种类细胞中稳定表达，尤其 … WebDec 5, 2024 · The conserved nuclear gene elongation factor-1 alpha (EF-1α) has been valuable as a higher-level phylogenetic marker in insects and it has also been widely used to investigate the evolution of intron/exon structure. To explore evolutionary relationships among Psylloidea, polymerase chain reaction amplification and nucleotide sequencing of … WebThus, the sequences of the EF1α-1 and EF1α-2 promoters are quite similar, except for the 81 bp SV40 small antigen sequence at the 5′-end of EF1α-2. dating on christian mingle

The human elongation factor 1 alpha (EF-1α) first intron …

Ef-1α intron a的功能

Phylogenetic Utility and Evidence for Multiple Copies of …

WebLentiviral PrecisionShuttle™ vector with a puromycin resistance marker, for expression driven by the EF-1α promoter of an ORF fused to C-terminal c-Myc and FLAG® tags. ... WebDriving Directions to Tulsa, OK including road conditions, live traffic updates, and reviews of local businesses along the way.

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Webofthepresenceofthewild-type(wt)EF-1α rstintroninthepromoter,whichresultsinaminorincreasein green … WebpBApo-EF1 (alpha) Neo. Simple mammalian expression vector with an EF-1α promoter and a neomycin resistance marker. Sequence Author: TaKaRa. Open in SnapGene. Try …

WebMay 1, 2001 · The β-tubulin gene intron 3 region and EF-1α intron 4 should prove useful in phylogenetic and epidemiological studies of additional Claviceps species. Primers that allow direct PCR detection of C. africana from honeydew produced on infected sorghum plants, providing a useful tool for analysis of field samples are described, and should prove ... WebFeb 14, 2002 · We inserted the first intron of the human EF-1α gene behind the MCMV and the HCMV promoter to enhance the gene expression through increasing RNA …

WebAbstract. We have identified a cellular enhancer-binding protein, present in nuclear extracts prepared from human and rodent cells, that binds to the adenovirus E1A enhancer … WebJun 23, 2024 · The EF-1α promoter exhibits a strong activity and yields long lasting expression of a transgene in vivo. The R-U5’ has been coupled to the EF-1α core promoter to enhance stability of RNA" Cite

WebJun 1, 2024 · Mitochondrial, nuclear, and endosymbiont DNA sequences of 12 introduced populations from three continents confirm they all belong to Essigella californica (Essig, 1909). Intron sequence variation of the nuclear gene EF-1α has revealed the existence of four distinct groups.

WebNov 30, 2005 · We recommend a suitable focus region of 500 bp for future studies of EF-1α in Hexapoda. > Key words Elongation factor-1 alpha, exon-intron structure, intron gain, intron loss, sequenced regions ... dating on craigslistWebJul 16, 1990 · It appeared that the first intron sequence in oyster EF-1α gene was required for the promoter activity. DNA construct without the first intron located upstream of the ATG start codon failed to drive EGFP expression in oyster embryos. Collectively, these studies indicate that the endogenous EF-1α promoter is preferred promoter for driving gene ... dating on craigslist goneWebApr 1, 2004 · Intron positions in EF-1α from well-studied arthropod taxa, modified from Danforth and Ji (1998) and Jordal (2002). Bees, beetles, and Drosophila possess two paralogous copies of the gene. The map shows only coding regions and includes the maximum length sequenced from each taxon. Known intron locations are marked with … dating on demand chicagoWebAccording to these findings, we concluded that the EF-1α promoter is a potent regulatory sequence for episomal vectors because it maintains high transgene expression, … dating on american idolWebDec 4, 2024 · The expression of multiple genes requires ribosomal entry or skip sites, or the use of multiple promoters. Promoter systems comprised of two separate, divergent promoters may significantly increase the size of genetic cassettes intended for use in gene therapy. However, an alternative approach is to use a single, compact, bidirectional … bj\u0027s brewhouse corporate office phone numberWebIn this study, we investigated the effect of five different introns on transgene expression in CHO cells. hCMV intron A, adenovirus tripartite leader sequence intron, SV40 intron, Chinese hamster EF-1alpha gene intron 1 … dating on cruise shipsWebeIF1A（eukaryotic translation initiation factor 1A，真核起始因子1A）是一種在真核翻譯起始進程中發揮重要作用的蛋白質，也是一種RNA結合蛋白。其主要的功能是穩定Met … dating on cape cod